(NAS Colloquium) Virulence and Defense in Host--Pathogen Interactions Common Features Between Plants and Animals (2001) / Chapter Skim
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AvrPto-dependent Pto-interacting Proteins and AvrPto-interacting Proteins in Tomato
AvrPto-dependent Pto-interacting Proteins and AvrPto-interacting Proteins in Tomato
Pages 85-89
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From page 85... ...
Martin* Boyce Thompson Institute For Plant Research and Department of Plant Pathology, Cornell University, Ithaca, NY 14853 The plant-intracellular interaction of the avirulence protein AvrPto of Pseudomonas syringae pathovar tomato, the agent of bacterial speck disease, and the corresponding tomato resistance protein Pto triggers responses leading to disease resistance.
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From page 86... ...
Individual yeast transformants expressing each of the indicated three- or two-hybrid protein combinations were streaked to minimal medium agar plates containing 40 ,ug/ml X-gal to assay expression of the lacZ reporter gene, indicated by a developing blue color, and to minimal medium lacking leuci ne to assay expression of the /eu2 reporter gene, i nd icated by growth. The Drosophila proteins Bicoid and Dorsal were used as arbitrary bait and prey fusions (kindly provided by R
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From page 87... ...
Isolation and Characterization of Proteins That Interact with AvrPto A standard AvrPto bait construct (C-terminal fusion of AvrPto to the LexA DNA-binding domain) made previously in our laboratory was found to activate both the leu2 and the lacZ reporter genes in the absence of prey (R.
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From page 88... ...
of the tomato cDNA library was conducted by using pBOG10. Thirty million primary transformants, 1,480 leucine prototrophs, and ultimately 58 clones showing strong lacZ activation and 94 clones showing weak lacZ activation were obtained.
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From page 89... ...
Ultimately, we hope, further characterization of the Adi and Api proteins will yield a yet more detailed understanding of the molecular mechanisms involved in bacterial speck disease and disease resistance, and contribute toward the development of means to control this disease, and others, through genetic manipulation. We thank Elizabeth Suryaatmaja and Philip Pusey for assistance conducting the yeast screens and Yuko Nakajima for specificity screening of AdiS and helpful discussion.
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