Immunodeficient Rodents A Guide to Their Immunobiology, Husbandry, and Use (1989) / Chapter Skim
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4 MAINTENANCE OF RODENTS REQUIRING ISOLATION
4 MAINTENANCE OF RODENTS REQUIRING ISOLATION
Pages 148-154
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From page 148... ...
result in an immunodeficiency that is so severe that exposure of these animals to agents of even relatively low pathogenicity results in severe illness and death. For example, several studies have shown that athymic mice exposed to mouse hepatitis and Sendai viruses have survival times of less than 3 weeks (Sebesteny and Hill, 1974; Ward et al., 19761.
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From page 149... ...
The committee recommends the use of transparent plastic cages to facilitate routine animal observation without the need to open the cage more frequently than is necessary for sanitation and experimentation. These units represent the simplest solution to housing pathogen-free rodents, but they require rigid discipline and operating procedures if they are to be used as a primary barrier against disease (Sedlacek et al., 19801.
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From page 150... ...
Germfree Isolators For complete exclusion of microbes, the ideal housing system is the pos itive pressure isolator, such as those used to house germfree, or gnotobiotic, rodents. The most widely used isolators are made of flexible laminated vinyl plastic (ILAR, 1970, pp.
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From page 151... ...
Tn~livi~luallv Ventilated Cage Racks _ . ~ ,,, Ventilated racks that accommodate cages in enclosed cabinets or by suspension under closed tops and that supply air under positive or negative pressure or both are available.
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From page 152... ...
As a practical matter, these steps are rarely taken. Furthermore, excessively high room temperatures create husbandry problems, such as fermentation of feed and bedding, excess bacterial growth in watering systems, and an unpleasant work environment.
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From page 153... ...
In the case of barrier rooms operated as maintenance facilities for rodents from outside suppliers, the opportunities for failure exceed those of carefully managed, closed-colony facilities with integral breeding. In this case, it is essential that suppliers provide complete documentation of the health status of their colonies and that they be required to notify users immediately if they experience a failure of the desired health status of their colonies.
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From page 154... ...
Therefore, an accurate evaluation requires that both immunodeficient and immunocompetent rodents be tested for the viral status of the colony. Immunocompetent sentinel rodents should be distributed randomly in a room that houses immunodeficient rodents, and a single individual from each cage of sentinels should be examined periodically for infectious diseases.
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