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Immunodeficient Rodents A Guide to Their Immunobiology, Husbandry, and Use (1989) / Chapter Skim
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5 MATING SYSTEMS FOR MUTANTS
Pages 155-160

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From page 155...
... At least 20 or more generations of consecutive brother x sister matings constitute an inbred strain. However, when expanding a strain beyond the F20 generation, all future matings must come from a common ancestor of at least the F20 generation, and, as inbreeding continues, the common ancestor should be kept as close as possible to the most advanced generation.
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From page 156...
... Inbreeding a Balanced Stock Closely linked mutant genes can be used to distinguish heterozygotes of lethal or sterile recessive mutants. Because these lethal or sterile mutants must be bred from heterozygotes, there is a considerable saving of cage space
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From page 157...
... , which is about 1 cM away on chro mosome 4. Closely linked marker genes can also be used in coupling (both mutant alleles are on the same chromosome)
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From page 158...
... Cross-lntercross Matings Using Ovarian Transplantation Cross-intercross matings using ovarian transplantation are used to maintain lethal or sterile recessive mutations. The ovaries of the homozygous mutant are removed at any time from 14 days to several weeks of age and are transplanted into the empty ovarian capsule of a histocompatible female host.
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From page 159...
... However, this is an expensive' and space-consuming process, and, although hybrid mutants and controls with known genotypes are produced, the breeding stock is inbred and is often very difficult to maintain. The second method is to transfer the mutation to a hybrid background that is made from two standard inbred strains and that is also color coded to permit ovarian transplantation.
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From page 160...
... Cross-Intercross Matings Using Ovarian Transplantation Cross-intercross matings using ovarian transplantation can be accomplished using the compatible sets of hybrids just described. For example, the mutation osteopetrosis (op.)
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Key Terms

  • brother x
  • x sister
  • ovarian transplantation
  • matings using
  • selected inbred

  • sister matings
  • inbred strains
  • standard inbred
  • using ovarian
  • inbred background

  • sterile recessive
  • hybrid background
  • recessive gene
  • linked marker
  • sister inbreeding

  • backcross matings
  • strain produced
  • recessive mutation
  • seven crosses
  • mutant alleles

  • marker gene
  • recessive mutant
  • inbreeding brother
  • ensure histocompatibility
  • using heterozygotes

  • dominant gene
  • homozygous mutant
  • immunologic gene
  • accomplished using
  • double heterozygote


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