Biosafety in the Laboratory Prudent Practices for Handling and Disposal of Infectious Materials (1989) / Chapter Skim
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Appendix B: 1988 Agent Summary Statement for HIVs, Including HTLV-III, LAV, HIV-1, and HIV-2
Appendix B: 1988 Agent Summary Statement for HIVs, Including HTLV-III, LAV, HIV-1, and HIV-2
Pages 141-154
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From page 141... ...
the following units of the Centers for Disease Control: AIDS Program, Hospital Infections Program, and Office of the Director, Center for Infectious Diseases; the Training and Laboratory Program Office; and the Office of Biosafety, Office of the Centers Director. Representatives of the following organizations also collaborated in the effort: American Academy of Microbiology, American Biological Safety Association, American Society for Microbiology, American Society for Clinical Pathology, Association of State and Territorial Public Health Laboratory Directors, College of American Pathologists, Pharmaceutical Manufacturers Association, and Walter Reed Army Institute for Research.
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From page 142... ...
Laboratory directors, supervisors, and others are asked to attach a copy of this revised "1988 Agent Summary Statement for HIVs" to each copy of the Guidelines and to all copies of their laboratory biosafety manual; they should review the recommended precautions with laboratory personnel, provide appropriate training in practices and operation of facilities, and ensure that all personnel demonstrate proficiency BEFORE being allowed to work with HIV. The laboratory director (or the designated laboratory supervisor)
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From page 143... ...
Hand washing with soap and water immediately after infectious materials are handled and after work is completed-EVEN WHEN GLOVES HAVE BEEN WORN as described aboveshould be a routine practice. Human immunodeficiency virus has been isolated from blood, semen, saliva, tears, urine, cerebrospinal fluid, amniotic fluid, breast milk, cervical secretions, and tissue of infected persons and experi
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From page 144... ...
Many commercially available chemical disinfectants (5,2023) can be used for decontaminating laboratory work surfaces, for some laboratory instruments, for spot cleaning of contaminated laboratory clothing, and for spills of infectious materials.
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From page 145... ...
certain procedures in which infectious aerosols are created are conducted in biological safety cabinets or other physical containment equipment. The following standard and special practices, safety equipment, and facilities apply to agents assigned to Biosafety Level 2: A
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, a hazard warning sign that incorporates the universal biohazard symbol is posted on the access door to the laboratory work area. The hazard warning sign identifies the infectious agent, lists the name and telephone number of the laboratory director or other responsible personas)
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From page 147... ...
5. When infectious materials or infected animals are present in the laboratory or containment module, a hazard warning sign (incorporating the universal biohazard symbol)
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6. All activities involving infectious materials are conducted in biological safety cabinets or other physical-containment devices within the containment module.
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From page 149... ...
7. Special care is taken to avoid contaminating skin with infectious material; gloves should be worn when handling infected animals and when skin contact with infectious materials is unavoidable.
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4. The laboratory director or other responsible person limits access to the animal room only to personnel who have been advised of the potential hazard and who need to enter the room for program or service purposes when infected animals are present.
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From page 151... ...
. The exhaust air from the animal room that does not pass through biological safety cabinets or other primary containment equipment can be discharged to the outside without being filtered or otherwise treated.
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From page 152... ...
If the HEPA-f~tered exhaust air from Class I or Class II biological safety cabinets is discharged to the outside through the building exhaust system, it is connected to this system in a manner (e.g., thimbleunit connection) that avoids any interference with the air balance of the cabinets or building exhaust sys tem.
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From page 153... ...
CDC. Public Health Service guidelines for counseling and antibody testing to prevent HIV infections and AIDS.
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From page 154... ...
CDC. Additional recommendations to reduce sexual and drug abuse-related transmission of human T-lymphotropic virus type III/lymphad APPENDIX B enopathy-associated virus.
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