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Live Variola Virus Considerations for Continuing Research (2009) / Chapter Skim
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Appendix: Variola Strains Used to Validate Diagnostic and Detection Assays
Pages 139-152

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From page 139...
... Appendix Variola Strains Used to Validate Diagnostic and Detection Assays (Table on following pages.)
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From page 140...
... Real-Time PCR VAR HA genee N Real-Time PCR 14-kDa protein genef Y 66-39, CDC v66-39, Sao (Brazil) Real-Time PCR VAR HA genee N Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR 14-kDa protein geneh Y Real-Time PCR 14-kDa protein genef Y 68-59 (Benin)
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From page 141...
... Real-Time PCR VAR HA genee N Real-Time PCR pan-E9L3, VAR B9R, VAR 10R genesj N 73-225, BOT73_225, CDC v73-225 Real-Time PCR VAR HA genee N (Botswana) Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR 14-kDa protein genef Y 77-1252 (Somalia)
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From page 142...
... Real-Time PCR VAR HA genee N Real-Time PCR 14-kDa protein geneh Y Real-Time PCR 14-kDa protein genef Y Real-Time PCR pan-E9L3, VAR B9R, B10R genesj Y Aslam, S/ASL (Pakistan) Microarray VAR C33L/B29R, B19R genesa Y MPCRc five pairs of primersd Y Microarray CrmB geneb Y Aziz, S/AZI Microarray VAR C33L/B29R, B19R genesa Y Microarray CrmB geneb Y Variola major-ABA (Bangladesh)
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From page 143...
... Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR pan-E9L3, VAR B9R, VAR B10R genesj Y Butler, S/BUT (United Kingdom) Real-Time PCR VAR HA genee N Microarray VAR C33L/B29R, B19R genesa Y MPCRc five pairs of primersd Y Real-Time PCR 14-kDa protein genef Y Microarray CrmB geneb Y China Horn, CHN48_horn (China)
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From page 144...
... Real-Time PCR VAR HA genee N Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR 14-kDa protein genef Y Variola minor-GAR (Garcia) Real-Time PCR VAR HA genek N Variola major-HAR Real-Time PCR VAR HA genek N Harper (Japan)
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From page 145...
... Real-Time PCR 14-kDa protein genef Y Higgins, UNK47_hig (United Kingdom) Real-Time PCR VAR HA genee N Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR 14-kDa protein geneh Y Real-Time PCR 14-kDa protein geneh Y Helder, S/HEL Microarray VAR C33L/B29R, B19R genesa Y Hinden (United Kingdom)
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From page 146...
... Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR 14-kDa protein genef Y Juba (Sudan) Real-Time PCR VAR HA genee N Real-Time PCR 14-kDa protein genef Y K1629 (Kuwait)
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From page 147...
... Real-Time PCR 14-kDa protein genef Y Minnesota124 (United States) Real-Time PCR VAR HA genee N Real-Time PCR 14-kDa protein genef Y MS Lee Real-Time PCR VAR HA genee N Real-Time PCR 14-kDa protein geneh Y 147 continued
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From page 148...
... Real-Time PCR VAR HA genek N Rafiq, Lahore, PAK69_lah (Pakistan) Real-Time PCR VAR HA genee N Sequencing GeneChips whole-genome sequences of 24 VAR strainsg N Real-Time PCR 14-kDa protein genef Y Rumbec (Sudan)
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From page 149...
... Real-Time PCR VAR HA genee N Real-Time PCR 14-kDa protein genef Y Taj Barin, S/TAJ Microarray VAR C33L/B29R, B19R genesa Y Variola minr-BUT Real-Time PCR VAR HA genek N (United Kingdom) Yamada (Japan)
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From page 150...
... 2004. Smallpox and pan-orthopox virus detection by real-time 3′-minor groove binder TaqMan assays on the roche LightCycler and the Cepheid smart Cycler platforms.
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From page 151...
... 2005. Multiplex PCR detection and species differentiation of orthopoxviruses pathogenic to humans.
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Key Terms

  • protein genef
  • five pairs
  • crmb geneb
  • sequencing genechips
  • var strainsg

  • protein geneh
  • crmb genei
  • clinical microbiology
  • smallpox virus
  • sierra leone

  • south africa
  • variola virus
  • var strain
  • pcr assay
  • var strainsh

  • microarray contained
  • taqman assays
  • simultaneous identification
  • recognize human
  • uman tissue

  • archival storage
  • identify novel
  • fragment length
  • identify variola
  • china horn

  • pathogenic orthopoxviruses
  • kali mathu
  • cepheid smart
  • taj barin
  • roche lightcycler


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