Live Variola Virus Considerations for Continuing Research (2009) / Chapter Skim
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5 Genomic Analysis
5 Genomic Analysis
Pages 59-66
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From page 59... ...
Poxvirus genome sequence analysis can be used to identify potential targets for the development of therapeutics and vaccines, and offers the promise of revealing the molecular events underlying smallpox infection and pathogenesis and the host response. The last 10 years have seen enormous technological advances leading to much more rapid and much less expensive methods for genome sequencing.
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From page 60... ...
In work published in 2006, full genome sequences were determined for 43 geographically distinct VARV isolates held in the CDC repository. Several other variola genome sequences are also available at present, for a total of at least 49.
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From page 61... ...
Some relationships between variola genome sequences and disease severity have been identified, although these associations are only broadly defined at present because the range of case fatality rates within some clades is large. For example, the case fatality rates of isolates from Asian clade C range from <1 percent to 38 percent, compared with 8 percent to 12 percent for west African clade A and 0.8 percent for South American clade B
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From page 62... ...
; the Asian clade C includes a subgroup of non–west African African variants. Case fatality rates associated with some isolates are indicated in parentheses.
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From page 63... ...
In comparison with the significant effort required to determine a complete poxvirus genome sequence in 1993, the effort necessary today to sequence all remaining variola isolates would be relatively minor. In fact, if DNA were made available, current next-generation sequencing technology would enable the determination of complete genome sequences for all remaining variola strains in a total of several weeks by one laboratory, at a low cost.
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From page 64... ...
The latter need can be met either by cultivation and DNA harvesting or by DNA amplification methods, the products of which can be saved in the form of genomic clones or amplified DNA. On the other hand, live variola virus would be needed to perform functional studies (such as studying RNA or protein expression or host interactions)
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From page 65... ...
2007. Protein composition of the vaccinia virus mature virion.
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