Mitochondrial Replacement Techniques Ethical, Social, and Policy Considerations (2016) / Chapter Skim
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Appendix B: Summary of MRT Research
Appendix B: Summary of MRT Research
Pages 165-176
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and pronuclear transfer (PNT) (see Table B-2)
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C r yo - t h aw M S T o o c y t e s : (O H S U G ro u p) • Tra n s f e r o f f re s h s p i n d l e t o v i t r i f i e d c y t o p l a s t a n d v i c e ve r s a • I m p l a n t a t i o n o f f o u r b l a s t o c ys t s d e r i ve d by t ra n s f e r o f v i t r i f i e d s p i n d l e s i n t o f re s h o o c y t e Nonhuman primate cytoplasts (rhesus macaques)
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s u c c e s s f u l l y f u s e d p ro n u c l e i a n d • 9 f u s e d o o c y t e s ( 8 2 p e rc e n t ) s u c c e s s f u l l y ex t r u s i o n o f s e c o n d fertilized polar body)
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o o c y t e s a n d z yg o t e s , re s p e c t i ve l y
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APPENDIX B 169 Endpoints Results • O ve ra l l h e a l t h a n d • L i ve b i r t h o f a f e m a l e o f f s p r i n g (C r y s t a ) p o s t n a t a l d eve l o p m e n t of rhesus macaque 2009 rhesus offspring: o f f s p r i n g f ro m 2 0 0 9 • N o r m a l d eve l o p m e n t study • N o c h a n g e i n h e t e ro p l a s m y i n b l o o d a n d s k i n samples • D eve l o p m e n t a l • S i g n i f i c a n t p ro p o r t i o n o f M S T o o c y t e s ( 5 2 potential p e rc e n t )
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T h i rd s c i e n t i f i c rev i ew o f t h e s a f e t y a n d e f f i c a c y o f m e t h o d s t o a vo i d m i t o c h o n d r i a l d i s e a s e t h ro u g h a s s i s t e d c o n c e p t i o n : 2 0 1 4 u p d a t e . NOTE: ESC = embryonic stem cell; F1 = first generation; F2 = second generation; ICSI = intracytoplasmic sperm injection; IVF = in vitro fertilization; MII = metaphase II; MST = maternal spindle transfer; mtDNA = mitochondrial DNA; ND = non-detectable; NS = non-significant; PNT = pronuclear transfer; SeV = Sendai virus; TE = trophoectoderm.
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− P N T z yg o t e s : 0 .
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: second polar body biopsy C 57 B L /6 J ( B 6 ) w i t h • 3 9 P N T z yg o t e s , Δ m t D N A /t o t a l m t D N A e s t i m a t e d 4696-bp deletion t o b e 1 7- 5 3 p e rc e n t ( ave ra g e 2 5 p e rc e n t )
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2 p e rc e n t o f 1 - a n d • E m b r yo n i c 2 - p ro n u c l e i t ra n s f e r P N T z yg o t e s , re s p e c t i ve l y, d eve l o p m e n t a l d eve l o p e d p a s t 8 - c e l l s t a g e ; 8 . 3 p e rc e n t t o potential b l a s t o c ys t s t a g e ( 5 0 p e rc e n t o f n o n m a n i p u l a t e d , a b n o r m a l l y f e r t i l i z e d c o n t ro l s)
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T h i rd s c i e n t i f i c rev i ew o f t h e s a f e t y a n d e f f i c a c y o f m e t h o d s t o a vo i d m i t o c h o n d r i a l d i s e a s e t h ro u g h a s s i s t e d c o n c e p t i o n : 2 0 1 4 u p d a t e . NOTE: ∆mtDNA = mitochondrial DNA deletion; F1 = first generation; F2 = second generation; MII-SCC = metaphase II-spindle chromosome complex; MST = maternal spindle transfer; mtDNA = mitochondrial DNA; ND = non-detectable; NS = non-significant; PNT = pronuclear transfer.
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− P N T z yg o t e s : 0 .
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2014. Polar body genome transfer for preventing the transmission of inherited mitochondrial diseases.
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