Stemming the Tide Controlling Introductions of Nonindigenous Species by Ships' Ballast Water (1996) / Chapter Skim
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H EMERGING MONITORING TECHNOLOGIES
H EMERGING MONITORING TECHNOLOGIES
Pages 122-126
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From page 122... ...
The ATP content of cells varies widely with cell size; therefore, ratios of nucleic acids or protein to cell size are perhaps most representative of population viability. Such ratios would need to be generated through research efforts for standardized monitoring of ballast water microbial populations.
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From page 123... ...
FLOW CYTOMETRIC TECHNIQUES A promising technique that could eventually be applied to monitoring ballast water for unwanted biological organisms is flow cytometry. This technique uses a modification of a typical Coulter counter system and can be operated in a flowthrough mode.
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From page 124... ...
Because of its specialized nature, the main drawback to the use of flow cytometry is expense, and unless a simplified version becomes available it is not clear that ships would voluntarily use such equipment on board. OTHER AUTOMATED TECHNIQUES Other possible procedures that may be adapted for automated shipboard use to determine the quality of ships ballast water are immunofluorescence, specific DNA probes, and extractable lipid phosphate analyses.
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From page 125... ...
1992. Partial rRNA sequences in marine yeasts: A model for identification of marine eukaryotes.
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