Amphibians Guidelines for the Breeding, Care and Management of Laboratory Animals (1974) / Chapter Skim
Currently Skimming:
III Definition and Description of Experimental Amphibians
III Definition and Description of Experimental Amphibians
Pages 27-38
The Chapter Skim interface presents what we've algorithmically identified as the most significant single chunk of text within every page in the chapter.
Select key terms on the right to highlight them within pages of the chapter.
From page 27... ...
Wild-caught nonconditioned treated (3) Wild-caught nonconditioned miscellaneous b.
|
From page 28... ...
Non trea ted (a) General Description Wild caught nonconditioned nontreated pre- or postmetamorphic amphibians refers to those collected in nature and shipped to the user with no handling or treatment other than that involved in catching, shipping to distribution points, holding between capture and sale and sorting, etc.
|
From page 29... ...
pipiens that cannot be ovulated in this season and are collected south of the ice line. Dealers must be most cautious con cerning admixture of these animals; their reproductive cycle is not the only physiological difference between them.
|
From page 30... ...
(2) Treated Wild~aught nonconditioned treated are animals that meet all of the criteria for the wild-caught nonconditioned nontreated classification and usually are provided some food prior to use or shipment to a buyer.
|
From page 31... ...
Standard Laboratory-reared standard are amphibians reproduced in the laboratory with at least one wild~caught parent or have been fed living food items collected from nature or living food items exposed to intermediate parasite hosts isee Chapter V, Section C.2 and Chapter VI, Section B.1 .b(2~]
|
From page 32... ...
Standard Laboratory-bred standard amphibians are those produced by reproductive events that did not occur in nature and whose parents were not field collected; they are fed processed food items or living food items bred under laboratory conditions isolated from the amphibian population. Thus, laboratory-bred amphibians must fulfill all the criteria required of laboratory-reared amphibians and must be at least of the F2 generation.
|
From page 33... ...
b. Heterozygous Isogenic Clones Groups of animals produced from wild-caught animals or random mating lines by the technique of nuclear transplantation (Chapter VII, Section A.9)
|
From page 34... ...
They may be produced either by a sequence of selected biparental matings or by any of the various techniques of parthenogenesis. Sequential biparental matings of amphibians yield inbred lines comparable to those common to inbreeding within over kinds of organism.
|
From page 35... ...
Homozygous diploids may be produced by transplanting haploid nuclei to enucleated eggs (see Chapter V'II, Section Am. A delay in cytokinesis occurs spontaneously in only a few eggs receiving transplanted nuclei.
|
From page 36... ...
and in the biparental matings of frogs from different geographical areas (1 9: 17.4 ~ from northern females X Mexican amelanoid males)
|
From page 37... ...
Other lines are in production or may be started by the investigator using the random mating or marked lines. The heterozygous marked lines are being developed using the best known mutants of R
|
From page 38... ...
. Heterozygous marked lines, mutant lines, and inbred lines have been developed.
|
Key Terms
This material may be derived from roughly machine-read images, and so is provided only to facilitate research.
More
information on Chapter Skim is available.